Influence of salicylic acid and phenylalanine on callus induction and tropane alkaloids production on Atropa belladonna In vitro

	Influence of salicylic acid and phenylalanine on callus induction and tropane alkaloids production on Atropa belladonna In vitro
Magazine of Al-Kufa University for Biology
Article 1, Volume 3, Issue 2, 2011, Pages 170-185
Abstract
An experiment was conducted in the tissue culture lab . College of Agriculture –University of Baghdad during 2009 - 2010 .Callus induction was achieved by culturing shoot tip of Atropa belladonna seedling on MS medium supplemented with 1.5 mg / l of 2,4-D and 0.4 mg / l of BA .Salicylic acid (SA) concentrations that added to MS medium were (0 , 1 , 2 , 3 ) mg / l and phenylalanine concentrations that added to MS medium were (10 , 20 , 30 , 40) mg / l . MS medium without SA gave the highest values (753.9 , 65.53) mg of both fresh and dry weights of callus, respectively .However values of both parameters were reduced with increasing SA concentration up to 3 mg/l (268.7 ,17.25) mg, respectively .Results indicated that MS medium supplemented with 20 mg/ l of phenylalanine gave the highest values of callus fresh and dry weights ( 584.2 , 48.84) mg, respectively .MS medium supplemented with (0 , 20) mg / l of SA and phenylalanine gave the highest values (938.2 ,83.25)mg of both fresh and dry weights of callus respectively.MS medium supplemented with {(2,40) ,(1,30) ,(1,20),(2,30)}mg /l of SA and phenylalanine, respectively gave the highest values (108.25 ,380.18,392.04 ,390.94) µg /g dry weight from callus of Atropine , Scopolamine , Tropine ,7-OH-Hyoscyamine, respectively.

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