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Purification and Characterization β - lactamase produce from local isolate Klebsiella pneumonia | ||
| Baghdad Science Journal | ||
| Article 1, Volume 6, Issue 1, 2009, Pages 50-60 | ||
| Authors | ||
| Munatha A. Al-Safar; Zuher A. S. Al-Taeei; Essam F. Al-Jumaily | ||
| Abstract | ||
| Beta-lactamase was purified from local isolate Klebsiella pneumonia by several steps included precipitation with ammonium sulphate at 20-40% saturation, DEAE- ion exchange chromatography and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 32.66; 47.04% respectively. The characterization of the purified beta-lactamase showed that the molecular weight was about 4000 daltons as determined by gel filtration. Purified enzyme had an optimal pH of 7 for activity and an optimal stability between pH 6.5-7.5, results shows that the optimal temperature appear to be 35 ه C . During storage the enzyme retained 72% at -20 ه C and retained 25% of the activity at the same period at 4 ه C. | ||
| Keywords | ||
| Lactamaiz; characterization Enzyme; get filtration; storage activity | ||
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