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Inhibitory effect of Hibiscus sabdariffa L.& Tamarindus indica against some pathogenic bacteria | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 13, Issue 3, 2008, Pages 163-172 | ||
| Author | ||
| Nawar Talal saffawi | ||
| Abstract | ||
| this study has involved the antibacterial activity of different concentrations of extracts of Hibiscus sabdariffa L.& Tamarindus indica has been tested against five species of pathogenic bacteria including: Staphylococcus aureus,Proteus vulgaris, Pseudomona, aeruginosa, Klebsiella pneumonia, Bacillus subtilis by using agar diffusion technique comparing with antibiotics. All bacteria showed response against extracts with different ratios. Klebsiella pneumonia showed maximum response of all extracts then followed by the other kinds of bacteria. The ethanolic extract constituents of both plants were isolated by using acid hydrolysis and ethylacetate. The identification of some active complexes isolated from ethanolic fraction was carried out by using thin layer chromatography (T.L.C) technique. Some acids isolated from ethanolic fraction like Malic acid and Tartaric acid were identified by using (T.L.C) technique by comparing its rates of flow (Rf) with the value of reference tables. Inhibitory effect of these isolated acids upon the germs were examined by using agar diffusion method. Moreover, the Minimum Inhibitory Concentration (MIC) of plant extracts under study and the isolated acids amounted between the (2.0-0.0625) mg/cm3. . | ||
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