Mechainism of Resistance  Staphylococcus sciuri Isolated from Cases  of Bacteremia and Endocarditis and Kidney Failure to Antibiotics

Abodi Ali, Fattma

	Mechainism of Resistance Staphylococcus sciuri Isolated from Cases of Bacteremia and Endocarditis and Kidney Failure to Antibiotics
Tikrit Journal of Pure Science
Article 1, Volume 11, Issue 2, 2006, Pages 97-111
Author
Fattma Abodi Ali
Abstract
This study involved the isolation and identification of Staphylococcus sciuri suspected to be infected with Bacteremia, endocarditis and kidney failures cases in hospitals of Mosul city. The study started from Feb. (2004) till Feb. (2005). A total of 202 blood samples were collected from both sexes. The bacteria were diagnoised using biochemical and physiological tests. The results recovered 16 isolates of Staph. sciuri . As concern antibiotics sensitivity test a variable rate between strain were observed, also the results showed that the strains posses predominant resistance differences between (6-24) antibiotics for Staph. sciuri .As regard the mechanism responsible for antibiotic resistance showed that 50% of Staph. sciuri was produced Extend Spectrum Beta lactamase (ESBLs) after using electrophoresis technique using Polyacrylamide gel with sodium dodocyl sulfate (SDS – PAGE) for BLs extracted from the 5 strains produced it and it revealed (1-3) bands with m.wt. between (20-26) KDa. For investigating the genetic factors responsible for antibiotics resistance for Staph. sciuri transformation experiments were carried out with standard strains Escherichia coli JM83 using DNA plasmid purified from 5 strains of Staph. scriui for the location of the antibiotic resistant gene.As regard responsible for It was found that this genes are located on plasmid DNA molecules and these results were supported by electrophoresis of DNA purified from plasmid transformed colonies of laboratory strains of E. coli JM83, a single band of plasmid DNA on agarose gel appeared to have between (1-4) bands according to the types of the strains. On the other hand, one strain couldn't give any band which may indicate the occurrence of gene code for antibiotic resistance may be carried by the chromosome too. To support the genetic result the chemical materials and physical factors were used to eliminate the antibiotic resistance of bacteria isolates a chemical was used SDS which appear to have high effectiveness in curing the plasmid DNA, for the most antibiotics have a cure rate between (30-100)% followed by the dye acridine orange between (20-80)% then ethidium bromide between (24-60)% finally temperature was raised to 44C, the cure rate between (30-70)%.

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