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Gamma Ray Effect on Food Demand to Growth Factors as S. typhimurium Isolated from Clinical Cases in Mosul City | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 11, Issue 2, 2006, Pages 78-84 | ||
| Author | ||
| Abdalrazzaq Khidhir Mahmood | ||
| Abstract | ||
| Three hundred samples were collected from stool, blood and urine from suspected patients with typhoid fever and food-poisoning. They collected from mains hospitals of Mosul City. Diagnostic were performed using microscopic examination, biochemical and serological tests, the results were supported by using API 20E system test, and then was determined by Salmonella institution in Baghdad. Five bacterial isolates of Salmonella typhimurium were selected depending on source of isolation and irradiated with the doses of (0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5) KGY, to determine the lethal dose for all S. typhimurium isolates. The percentage of antibiotic resistances of chloramphenicol, Nalidixic acid, Ampicillin, Trimethoprim, Streptomycin was differing with doses. After irradiation with the doses (1, 2, 3, 4) KGY the bacterial colonies that revealed losing their antibiotics resistance were tested for their demand to growth factors (Nitrogenous bases and vitamins). Bacterial colonies showed the needs to the nitrogenous bases (Thymine, Guanine, Cytosine, and Adenine) and vitamins (Thiamin, Nicotinic acid). It have been diagnosis isolated from S. typhimurium which were lose their resistant for the antibiotics and other acquired the resistant property for these antibiotics, also when nitrogenous bases and vitamins were isolate from minimal medium, colonies lost for their antibiotics resistant property were appear after the irradiation, this may be result to generating mutation spontaneous in some genotypes which is marcing the biological paths enzymes of growth factors as a result to Gamma rays effect. | ||
| Keywords | ||
| typhimurium | ||
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