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Study of Separation of Some Phenolic Compounds from Aloe vera Juice and Their Effect on Some Negative and Positive Gram Bacteria | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 11, Issue 1, 2006, Pages 214-218 | ||
| Author | ||
| Muthana J. Mohammad | ||
| Abstract | ||
| This study is designed for isolation and identification of some phenolic compounds from Aloe vera juice, and to determine the inhibitory effect of these compounds were investigated on the growth of some the bacteria, which include: Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, Salmonella typhi, Klebsiella pneumonia, Streptococcus pyogenes and Staphylococcus aureus. The fractionation process performed by using column chromatography over silica gel, infrared spectroscopy was used for identification of the isolated compounds which showed clear inhibitory effect on bacteria in comparison with control samples (Ciprofloxacin and Augmentin). The alcoholic extract for Aloe vera juice showed high effect on Ps. aeroginosa, it showed equal inhibiting effect on S. typhi and K. pneumonia in comparison with control samples, while the effect of the extract on E. coli and Staph. aureus less than the control sample, and finally no effect of the extract on Prot. mirabilis and Strept. pyogenosa. The phenolic compounds showed different inhibiting effect on bacteria under study, it showed high effect on all bacteria under study except Prot. mirabilis which no effect anti in comparison with control samples. When we compared the effect of alcoholic extract with effect of phenolic compounds on inhibited of bacteria growth, we showed the effect of the last more than another in comparison with control samples (Augmentin and Ciprofloxacin). | ||
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