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The evaluation of activity and asymetrieal isolation of Adenosine Diaminase in the healthy patints with Anemia and Rheumatiod Arthritis serum and the both diseases | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 11, Issue 1, 2006, Pages 159-163 | ||
| Author | ||
| Abid-Almunam Hamad Majeed | ||
| Abstract | ||
| Adenosine deaminase is one of the important enzymes that catalysts the salvage purin base. The effect of divalent metals ( Mn+2 ,Ca+2 ,Fe+2 ,Cu+2, Co+2 , and Cd+2 ) on activity concentration(O.O1M) was studied. The result showed the metals (Mn+2, Ca+2 and Fe+2) have activation effect to ADA while the (Cu+2, Co+2 and Cd+2) have an inhibition effect tothe enzyme in normal and abnormal seum in addition to ADA isoenzymes. The present study showed that (Cd+2) is the potent inhibitor to ADA activity. ADA isoenzymes in normal and abnormal serum are isolated by using three types column chromatography (Sephadex G1OO, G200, Cellulose- DEAE).The result, there are two isoenzymes for normal, anemic and patient with Rheumatoid Arthritis serum (ADA1,ADA2) while there are three isoenzymes in serum in both. | ||
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