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Secretion ß-lactamase Enzyme from some Gram negative Bacteria Causing Chronic Otitis Media disease In Nassriya | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 11, Issue 1, 2006, Pages 113-116 | ||
| Author | ||
| Majida ghazi magtooph | ||
| Abstract | ||
| Bacterial isolates Pseudomonas aeruginosa, Proteus mirabilis ,Proteus vulgaris and Escherichia coli ] were collected from patients with chronic otitis media in Nassriya city from 7/2004 to 12/2004 and tested for antibiotic sensitivity test using Baur-Kirby method and test the ability produce ß-lactamase using Ampicillin resistance isolates by direct capillary tube method. From forty isolates of pseudomonas aeruginosa,32 isolates were resistant to Ampicillin and just 25 isolates produce ß-lactamase enzyme. From 21 isolates Proteus mirabilis, 18 isolates were resistant to Ampicillin and just 11 isolates produce Enzyme. From 13 isolates Proteus vulgaris ,11 isolates were resistant to ampicillin and just 8 isolates produce ß-lactamase enzyme. The finally from 26 isolates of E. coli all were resistant to Ampicillin just 21 isolates produce ß-lactamase Enzyme. Estimation of enzyme production with time showed that during the first 5 minutes . six isolate of pseudomonas aeruginosa , one isolates from proteus mirabilis , two isolates of Proteus vulgaris and six isolates of E- coli but after 15 minutes , 10, 5 , 0 and 4 isolates produce this enzyme respectively. | ||
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