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Validation of an Eiectrometeric Method for Cholinesterase Measurement in the Plasma of Ducks | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 11, Issue 1, 2006, Pages 103-106 | ||
| Author | ||
| K. S. ABASS | ||
| Abstract | ||
| This research is designed to describe and validate electrometric method for measuring cholinesterase activity (ChE) in the plasma of 30-60 days old ducks. A suitable (ChE) assay mixture contain 0.2 ml of plasma , 3ml of distilled water , 3ml pH 8.1 buffer and 0.12ml of 7.5% acetylcholine iodide as the substrate .The one step incubation was for 30min. at 37Cه was followed . Acetylcholine chloride was also a suitable substrate for the described procedure .The estimated level of true (ChE) activity in the plasma after inhibition of pseudo (ChE) by quinidine was 27%. The organophosphate insecticide parathion (0.5-8 µM) and carbamate insecticide aldicarb (5-40µM) significantly inhibited plasma (ChE) activites in vitro. Oral administration of parathion (2 and 4 mg/kg) and aldicarb (100 and 200 mg/kg) ducks induced a characteristic in toxication signs of cholinergic over stimulation and these sign were associated with significant decreases in plasma (ChE) activites .The results suggested that the present electrometric method for measuring of (ChE) activities in ducks is simple , accurate and efficient in monitoring the enzyme inhibition caused by organophosphate and carbamate insecticides. | ||
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