- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Partial Purification of 5´-Nucleotidase Isoenzymes from Blood Sera of Anemic Patients | ||
| Rafidain Journal of Science | ||
| Article 7, Volume 20, Issue 2, 2009, Pages 81-92 PDF (0 K) | ||
| DOI: 10.33899/rjs.2009.40198 | ||
| Authors | ||
| Sabah H. Khorsheed; Ferah G. Al-Salihi; Asraa Yaseen | ||
| Department of Chemistry College of Education for Girls Tikrit University | ||
| Abstract | ||
| In this study, 5´-Nucleotidase isoenzymes were partially purified from blood sera of anemic patients, through different steps including: Protein precipitation with ammonium sulfate and dialysis. Three isoenzymes were isolated from dialyzed protein fraction by using Anion-exchange chromactography (DEAE-Cellulose A-50), with maximum purification folds (22.13) for isoenzyme Ш. In addition, the kinetic studies for the isolated isoenzymes were carried out, which included the effect of different substrate (5´- AMP) concentration and determination their Km values: 14.08, 12.98 and 8.4 (mM) respectively compared to normal; 13.0, 14.08 and 15.6 (mM). | ||
|
Statistics Article View: 400 PDF Download: 490 |
||