Detection of AmpC-β-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in Najaf Hospitals

	Detection of AmpC-β-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in Najaf Hospitals
Magazine of Al-Kufa University for Biology
Article 1, Volume 4, Issue 1, 2012, Pages 93-104
Abstract
Pseudomonas aeruginosa is of the major causes of hospital acquired infection. The isolates have often been observed to display considerable resistance to multiple antibiotics, hence the aim of this study is to identify the dissemination of AmpC β-lactamase in clinical isolates of this bacteria. A total of 37 Ps. aeruginosa isolates were obtained from Najaf hospitals. The isolates were tested for their antibiotic resistance against 16 types of antibiotics. All the isolates were found to be resistant to a minimum of 3 classes of antibiotics, hence, the isolates were considered multidrug resistant. The primary antibiotic susceptibility test revealed that 10 (27%) isolates were cefoxitin resistant. These isolates were tested for their ability to produce AmpC β-lactamases using two methods, AmpC disk and modified three dimensional, also examined for the presence of blaAmpC gene. The AmpC enzymes production was confirmed in only 4 isolates. These isolates were examined for the presence of some extended-spectrum β-lactamases genes. Results revealed that 1 isolate carried blaSHV, 1 isolate carried blaSHV, blaTEM and blaCTX-M and 1 isolate carried blaSHV and blaCTX-M. According to the results carried out in this study, the AmpC producing Ps. aeruginosa represent a serious therapeutic challenge.

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