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Estimation of cellular immune response by evaluation of some cytokines in immunopathogenesis of chronic hepatitis B and C pre- and post- treated Iraqi patients (in vivo and in vitro) | ||
| Baghdad Science Journal | ||
| Article 1, Volume 6, Issue 3, 2009, Pages 533-541 | ||
| Authors | ||
| Nawal Mahdi Al-khalidi; Ghanima Sadiq Mohammed; Raghad Harbi Al-azzawi | ||
| Abstract | ||
| Two groups of chronic hepatitis B and C virus patients were divided into Pre-treated patients (25 CHB patients with positive HBs Ag for more than 6 months and 40 CHC patients), and post-treated patients [12 CHB patients (4, 6, and 2 were treated with lamivudine, IFN-α and combination of LMV + IFN-α respectively), and 27 patients for CHC (3, 13 and 11 patients were treated with Ribavirin, IFN-α and combination therapy (RBV+ IFN-α) respectively].These patients were followed up for 6 months. By using ELISA technique, levels of IL-6, IL-10, IFN-γ and TNF-α were measured in vivo and in vitro (supernatant of PBMCs stimulated with PHA) and compared with healthy control. The mean level of IL-6, IL-10 and TNF-α in CHB patients showed significant differences (P<0.05) between pre- and post-treated patients in vivo and in vitro, while there was no significant difference in IFN- γ between pre- and post-treated patients in vivo and in vitro. The difference between control and CHB patients was highly significant (P<0.0001) in IL-6 , IL-10 and TNF- α levels in vivo and in vitro. In CHC patients the mean levels of IL-6, IL-10, IFN- γ and TNF- α showed significant difference between pre- and post-treated patients in vivo and in vitro. There was highly significant difference (P<0.0001) between patients and control in IL-10 levels. Hence, these observations indicate the predominance of Th2 cytokine, which promote the persistency of the CHB and CHC virus. | ||
| Keywords | ||
| cellular immune response; cytokines; chronic hepatitisB; chronic hepatitisC; IFN; TNF | ||
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