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Effect of administration of Levamisole and vitamin E on the immune response for vaccinated Guinea Pigs by Rev-1 vaccine | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 4, Issue 2, 2011, Pages 170-179 PDF (203.38 K) | ||
| Authors | ||
| M.N. Al-Saige; A. A. Yousif; A. K.Talal | ||
| Abstract | ||
| This experiment was designed to find out the influence of levamisole and vitamin E administration as immunopotentiater for Guinea pigs vaccinated with Brucella vaccine (Rev-1). Forty eight Guinea pigs were used (male and female) which divided into four groups as follows: First group were vaccinated with Brucella vaccine Rev-1 (1 ml contain 2x109 viable bacteria) subcutaneously in addition levamisole (10 mg/ kg B.W) was given orally at a week before vaccination, with vaccination then a week after vaccination, then at monthly intervals up to the end of the experimental period; Second group was given the vaccine at the same way of the first group with vitamin E at a dose of 100 mg / kg orally each time; Third group were given the vaccine orally, Fourth group considered as control group, and was given 1 ml of (PBS) S/C at each time. Clinical and immunological parameters studied for fourteen weeks. The results of this experiment revealed that a rise in body temperature, increase respiratory and pulse rates in vaccinated groups. no abortion was noticed in all pregnant animals while no changes were noticed in clinical signs of control group. The vaccine activated the humeral immunity in all vaccinated groups; Rose Bengal Test showed a positive results in all vaccinated groups in all times of experiment (each 4 week), while the Passive Haemagglutination Test gave a significant high level of antibody titer, in the first group 1066.66 followed the second group was 981.33 and third group 906.66 respectively, while the control group showed a negative result. Also the vaccine activate cellular immunity, the Delayed Type Hypersensitivity Test gave a positive result, on the first group which was significantly higher than the other two groups, while the control group showed negative result. E Rosette Test recorded high percentage of active 32% and total 36.54% lymphocyte; the first group showed the highest values then the second group and third group respectively. The vaccinated groups revealed a high total serum protein compared with control group, and the first group was 6.58 mg/dl significantly higher than the other two groups | ||
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