Study the antilisterial and immune stimulating effects of crud Listeria Lipids on Listeria monocytogenes

M. Al-Gburi, N.

	Study the antilisterial and immune stimulating effects of crud Listeria Lipids on Listeria monocytogenes
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 4, Issue 2, 2011, Pages 76-81 PDF (134.86 K)
Author
N. M. Al-Gburi
Abstract
In order to known the antilisterial, immune stimulating effects of crud Listeria Lipids (CLE) on Listeria monocytogene. crud Lipid Extract (CLE) were prepare, sensitivity test was don of this extract as antilisteria effect and comparing with antibiotics E15, C LR15, RA5, AM10, DO30, PY100 the result showed that the CLE of LM affect growth of LM and the inhibition zone 21.33±0.34 mm. comparing with antibiotics E15, C LR15, RA5, AM10, DO30, PY 10020.3±0.89, 19.66±0.33, 10.66±0.67, 23.33±0.89, 17.66±0.68, 24.66±0.34 respectively. The statical analysis has showed that there was asignifacans differences (p<0.05) between the antibiotics groups comparing with CLE and low with RA and highly with PY100. Also in order to known the effect of this crud Lipid on Listeria infection, 10 mice were immunized with CLE I/P (0.05 mg/0.5 ml) a twice dose with antervales period 14 days, after 10 days from second dose Delyed type hyper sensitivity test was done by skin test on foot pad with CLE 0.1 ml, then the thickening of foot pad measuring after 24 and 48h. then after 27 dayes of 1st dose mice were challenged with infected Lm, the result showed that the mean thickening of foot pad was, 1.6±0.048, 1.2±0.056 after 24,48 h. respectively. Result also showed decreased mortality rate 30% of immunized mice, while 100% of control and isolation of LM Liver, spleen and heart from the three immunized mice and all control which are died after 4-10 dayes that’s indicated the crud Lipid stimulate immune response that protect the mice.

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