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A simplified method for DNA extraction from human blood clot: A familiarized diagnostic tool in forensic analysis | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 5, Issue 3, 2008, Pages 395-399 | ||
| Authors | ||
| Israa Adnan Ibraheam; Sahib Ahmad; Muhammed Baqur | ||
| Abstract | ||
| We have simplified the conventional techniques used to extract DNA from clotted blood by salting out technique, in such away some simple chemical treatments (SDS and NaCl) of cellular lysate of clot substituted the hazardous organic solvents such as phenol. Distilled water played an important role in eliminating almost all RBCs contaminating the specimens. Differences were not identified between clotted blood procedure and non-clotted blood procedure. Therefore, this method might be used in routine clinical laboratories for forensic analysis such as finger printing and other tests. | ||
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