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OVIDUCTAL FLUSHING MEDIUM SUPPORT IN VITRO FERTILIZATION OF MICE :A BIOCHEMICAL AND HORMONAL STUDY | ||
| AL-TAQANI | ||
| Article 1, Volume 20, Issue 1, 2007, Pages 30-35 | ||
| Authors | ||
| Saad S. Al-Dujaily; Munaf S. Daoud | ||
| Abstract | ||
| The objective of the present study is to measure some biochemical and hormonal contents of oviductal flushing medium (OFM)used for in vitro fertilization and embryonic development of mice as a model for mammals. Oviductal flushing medium of superovulated and spontaneously ovulated mice was analyzed for certain biochemical and hormonal contents. Amino acids( taurine, alanine, aspartate, glutamine and glycine) were measured using HPLC. Glucose and fructose were estimated using glucose oxidase method and fructose indol-reagent colorimetric method ,respectively. Magnesium , calcium ,sodium and potassium ions were determined using flame photometry and atomic absorption spectrophotometry. Luteinizing hormone (LH) and follicular stimulating hormone(FSH) were measured by enzyme linked fluorescent assay technique. The results of this investigation show that there is no significant (P>0.05) difference in the concentration of taurine ,glutamine and alanine in the OFM of SUO and SO mice. The OFM contents of aspartate and glycine in SUO is significantly(P<0.05) higher than that of SO group .A significant (P<0.05) decrease was observed in the concentration of glucose (µmol/L) in OFM of SUO compared to SO females.The concentration of Ca++ in the OFM of SUO (66.5µg/ml) was significantly (P<0.05) higher than that of SO mice(60.2 µg/ml). There was a significant(P<0.05) decrease in the level of LH in the OFM of SUO compared to SO mice .It is concluded from the present study that the OFM of superovulated mice contains higher amounts of different amino acids ,electrolytes and hormones that lead to offer a proper environment for in vitro fertilization and embryonic development of early cleavage stages in mice . | ||
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