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Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System | ||
| Baghdad Science Journal | ||
| Article 1, Volume 8, Issue 3, 2011, Pages 728-735 | ||
| Author | ||
| Wisam H. Salo | ||
| Abstract | ||
| Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-β-galactosidase Control Vector which encoded β-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently. | ||
| Keywords | ||
| Reporter gene; Hep; transfection | ||
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