- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Effect of low temperature on Tropane Production in vitro and in vivo of Datura metel Mill | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 2, Issue 0, 2010, Pages 29-38 | ||
| Abstract | ||
| This work was conducted to determine the effect of low temperature of qualitatively and quantitavely of tropane alkaloids ( Hyosciamine and Hyoscine) in leaves of Datura metel and in leaves callus induced from leaves by using MS medium supplemented with 2,4-D at concentration of 2 mg/L. The effect of low temperature(10±1,5±1) on alkaloids production in callus tissues .The alkaloids content between callus and leaves of stock plant comparing was studied too. This study include continuous alcohol extraction by ethanol (80%) of leaves and by ethanol and ether at ratio 4:1(v/v) for callus extraction. Detection of tropane alkaloids were accomplished by Dragendorff and Myer tests and the content alkaloids were done by HPLC ( high performance liquid chromatography. The result revealed that the increased of and with domination of hyosciamine content on Hyoscine content. Also, the result Hyosciamine and Hyoscine content in callus of Datura metel with increase of low temperatures indicate that decreases of callus fresh and dry weight with the decreasing temperatures. | ||
| Keywords | ||
| Key Words; callus; MS medium | ||
|
Statistics Article View: 29 |
||