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SYNTHESIS AND CHARACTERIZATION OF NEW N-TERMINAL BCHAIN MODIFIED HUMAN INSULIN ANALOGUES | ||
| Journal of Al-Nahrain University - Science | ||
| Article 1, Volume 13, Issue 4, 2010, Pages 23-30 | ||
| Author | ||
| Jewad K. Shneine | ||
| Abstract | ||
| New human insulin analogues were synthesized by fragment condensation reactions of N A1, N B29 Msc protected native human insulin of different B-chain lengths with the dipeptide Boc-Asn- Thr-OH. Edman degradation was used to obtain shortened B-chain insulin intermediates. Purification of the products was achieved using gel filtration, ion exchange chromatography and RP-MPLC chromatography. Analysis of synthesized analogues accomplished by RP-HPLC chromatography and amino acid analysis. The modified insulin analogues with the amino acids Asparagine in the first position and Threonine in the second position of the N-terminal B-chain of insulin would be expected to show an enhanced T R* transition compared with that of wild-type structure. | ||
| Keywords | ||
| Insulin; Peptide Synthesis; R Transition | ||
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