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THE EFFECT OF DIFFERENT CULTURE MEDIA ON IN VITRO FERTILIZATION OF MICE | ||
| Journal of Al-Nahrain University - Science | ||
| Article 1, Volume 13, Issue 4, 2010, Pages 147-150 | ||
| Authors | ||
| Mohammed A. Ibrahim; Hazim I. Al-Ahmad; Wasnaa H. Mohammed | ||
| Abstract | ||
| The effect of different types of media on in vitro fertilization (IVF) of mice oocyte was investigated. IVF was carried out in RPMI-1640 medium at recommended temperature (37 C) and pH (7.4). The obtained results showed that 63.73% of used oocytes were fertilized, and the percentage of abnormal embryos was 27.58%. While, using other culture media at same temperature and pH, gave low percentage of fertilization and higher yield of abnormal embryos. Thus, using Medicult IVF, Earl s and TCM-199 were gave fertilization percentages equivalent to 38.75, 42.42 and 61.45% respectively; whereas, the percentages of abnormal embryos were 32, 35.7 and 33.89 % respectively. The reported results indicate that composition of various types of culture media have significant effect on the rate of fertilization and abnormal embryos. | ||
| Keywords | ||
| culture medium; in vitro fertilization; IVF | ||
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