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Humoral and cellular immune response against Escherichia coli in vivo | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 4, Issue 3, 2007, Pages 219-228 | ||
| Authors | ||
| J. Hassan; ; ; H. Al-Alwany; Hassan F. Naji | ||
| Abstract | ||
| Enteropathogenic E.coli type I (EPEC) was isolated from feces of 8 months age infant baby. The bacterium was characterized according to the morphological, biochemical and serological properties. Their killed and formalized antigens (somatic and flagellar) were separated and mixed with complete and incomplete Freund's adjuvant (sunflower oil) which injected in rabbits (Oryctolagus cuniculus). The results were revealed that the effect of E.coli antigens in humoral immunity which expressed with immediate hypersensitivity were reached by single radical immunodiffusion assay (SRID) to 16.6, 15.4 and 19.4 mm in diameter for killed, somatic and flagellar antigens, respectively, while the control rabbits did not show such changes. The killed, somatic and flagellar antigens were explained the changes in cellular immune response by delayed hypersensitivity reaction and the results by SRID assay were 19.8, 17.8 and 26 mm in diameter, respectively, for the above mentioned antigens, while the control group did not show such changes. The concentration of total protein was appeared higher in rabbits which were immunized with killed antigen compared with other groups which reached to 87.3 g/L. The immunoglobulins (Ig) concentrations of rabbits primed with E.coli antigens were higher in treated groups compared with control group, but the treated group primed with somatic antigen was higher than other groups which reached to 2203, 326.9 and 577.5 mg/dL for IgG, IgM and IgA, respectively. The concentrations of complement components C 3 & C 4 were higher in treated groups primed with somatic antigen compared with other groups which reached to 266.5 and 60.4 mg/dL, respectively. These results suggest that the above antigens of EPEC were stimulated the humoral and cellular immune response. | ||
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