EFFECT OF SUBINHIBITORY CONCENTRATIONS OF SOME ANTIBIOTICS ON SLIME FORMATION AND CRUDE EXOPOLYSACCHARIDE PRODUCTION BY klebsiella  pneumoniae

Sharif Alyasiri, Nisreen

	EFFECT OF SUBINHIBITORY CONCENTRATIONS OF SOME ANTIBIOTICS ON SLIME FORMATION AND CRUDE EXOPOLYSACCHARIDE PRODUCTION BY klebsiella pneumoniae
AL-TAQANI
Article 1, Volume 18, Issue 1, 2005, Pages 121-127
Author
Nisreen Sharif Alyasiri
Abstract
Highly viscous K.pneumoniae isolated from urine of patient suffering from urinary tract infection was cultured on media containing different concentrations of Lincomycine, Metronidazole and Ciprofloxacine. The results showed that growth of encapsulated K.pneumoniae in medium containing sub-MICs of Lincomycine (16-64)µg/ml progressively increased the viscous slime formation and the amount of crude exopolysaccharide execrated. There were no notable or significant differences in slime formation occurred after the addition of sub-MICs of Metronidazole (8-32)µg/ml but there was remarkable reduction in the amount of crude exopolysaccharide execrated. On the other hand growth of encapsulated K.pneumoniae in media containing sub-MICs of Ciprofloxacine reduce the viscous slime formation at (4&8) µg/ml and remarkably increased the amount of exopolysaccharide execrated but only at low concentrations of sub-MICs which is 2 µg/ml. Further concentrations of sub-MICs of Ciprofloxacine (4-8)µg/ml reduced the viscous slime formation but have only a little effect on crude exopolysaccharide production. Increasing the production of exopolysaccharide with Lincomycine and low concentrations of Ciprofloxacine without inhibiting bacterial growth may be useful in extraction of capsular exopolysaccharide for research and laboratory purposes such as vaccine production.

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