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Detection of Some Virulence Factors of Enterobacter Cloacae Isolated from Children Diarrhea Cases in Mosul | ||
| Rafidain Journal of Science | ||
| Article 3, Volume 18, Issue 6, 2007, Pages 23-31 PDF (0 K) | ||
| DOI: 10.33899/rjs.2007.42488 | ||
| Authors | ||
| Adeeba Y. Shareef; Sarab D. Sulayman | ||
| Abstract | ||
| Some virulence factors of Enterobacter cloacae which isolated from children diarrheal cases under six year of age were detected. Enterotoxins production were detected using the rapid and delayed skin permeability tests of rabbit and the suckling infant mouse. The rapid permeability and the suckling infant mouse test showed positive responses regarded as an index for the production of heat stable enterotoxin however the delayed permeability test was negative which was probably an indication for the absence of heat labile enterotoxin. The production of alkaline phosphatase and protease were detected in both cell free supernatant and lysate supernatant. The specific activities of both were 2.120 , 0.120 unit/cm3/min/mg protein in cell free supernatant. No activity were seen in lysate supernatant. This indicates the ability of this bacteria to produce these enzymes extracellularly. | ||
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