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Study of Proliferation Markers in Normal and Diseased Uterine Cervix | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 2, Issue 2, 2005, Pages 158-165 | ||
| Authors | ||
| Ali Hassan Altimimi; Layla Abdul Amir | ||
| Abstract | ||
| Carcinoma of the cervix is one of the most common malignancies. Papanicolaou (Pap) smear tests have reduced mortality by up to 70%. Nevertheless their interpretation is notoriously difficult with high false-negative rates and frequently fatal consequences. We have addressed this problem by using affinity-purified antibodies against human proteins that regulate DNA replication, namely Cdc6 and Mcm5. Our materials were obtained from 100 smears and 60 cervical biopsies specimens taken at colposcopy clinics. Twenty-seven biopsies showed high-grade squamous intraepithelial lesions (HSILs), and 14 showed low-grade squamous intraepithelial lesions (LSILs). Normal cervical tissue was obtained from age-matched patients undergoing hysterectomy for diseases unrelated to the cervix (n = 19). These antibodies were applied to sections and smears of normal and diseased uterine cervix by using immunohistochemical technique to detect abnormal precursor malignant cells. Antibodies against Cdc6 and Mcm5 stain abnormal cells in cervical smears and sections with remarkably high specificity and sensitivity. Proliferation markers Ki-67 and proliferating cell nuclear antigen are much less effective. The majority of abnormal precursor malignant cells are stained in both low-grade and high-grade squamous intraepithelial lesions. Immunostaining of cervical smears can be combined with the conventional Pap stain so that all the morphological information from the conventional method is conserved. Thus antibodies against proteins that regulate DNA replication can reduce the high false-negative rate of the Pap smear test . | ||
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