The object of this study was focused on transmission of Literia monocytogenes through layer body , the role of this birds in the spread of this bacteria and the effect of experimentally infection dose of the layer orally on bacteria transmitted to eggs ,egg components and internal organs . A total of 45 layer were distributed into three groups and each group to three replicates (15 layer per replicate) cosnsisted of T1 (control group free of infection) , T2 and T3 were orally experimentally infected by 1x105 and 1x1010 (CFU ml hen of L. monocytogenes .
Results indicated that .L. monocytogenes were isolated from ovary, oviduct, uterus, proventiculous, gizzard, heart, liver, spleen, intestine and cecum after 3, 6, 9, 15and 21 days of experimentally infection and the lowest isolated rate from T2 group compared with T3 group .Also , the bacteria were isolated from egg shell , shell membrane and egg albumen of eggs produced from T2 and T3 group layers after 3, 6, 9, days and the isolation rate decreased gradually from the three components of the egg after 15 days ,when as the isolation from yolk membrane , yolk fluid and germinal cell after 6 days of infection and decreased gradually after 21 days . |