- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Study of Some DNA Plasmid Characters Purified From Klebsiella pneumoniae Isolated from Various Human Infection | ||
| Rafidain Journal of Science | ||
| Article 10, Volume 16, Issue 10, 2005, Pages 85-92 PDF (0 K) | ||
| DOI: 10.33899/rjs.2005.43306 | ||
| Authors | ||
| Khalid D. Ahmed; Ayad H. Hasan | ||
| Abstract | ||
| Specimens of the isolated bacteria Klebsiella pneumoniae from various pathogenic cases in human (urine, pus, stool, sputum, cerebrospinal fluid) were studied. These specimence were classified into five groups of isolates according to their resistance to the antibiotics (ampicillin, tetracycline, chloramphenico, streptomycine, cephalexine). Some of the characters are studied for the plasmid DNA in these isolates, of these, the self-transmissible ability of the plasmid DNA molecules though conjugation.The plasmid DNA of the isolates (1, 2, 4) have the ability to transfer the antibiotic resistant genes to the laboratory strain of E. coli (JM83). Then the amplification of the plasmid DNA were studied in these isolates. Only the isolates (3, 5) have revealed high yield of purified plasmid DNA in the presence of chloramphenicol (100mg/ml). | ||
|
Statistics Article View: 361 PDF Download: 247 |
||