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Curing of Serratia marcescens Plasmid by High Temperature and by Acridine Orange and Urea | ||
| Rafidain Journal of Science | ||
| Article 18, Volume 16, Issue 14, 2005, Pages 191-199 PDF (0 K) | ||
| DOI: 10.33899/rjs.2005.43526 | ||
| Author | ||
| Bayda Gh. Mohammad | ||
| Abstract | ||
| The present study was conducted on five isolates of S. marcescens from septicemic patients in Al-Salam Hospital in Mosul. Isolates were identified by using cultural and biochemical characteristics as well as the Api 20E system Resistance of the isolates to Chloramphenicol, Tetracycline, Ampicillin, Erythromycin and Gentamycin was checked. Curing the Isolates of their plasmids was performed by using high temperature (45c), acridine orange (40 g/ml) and urea (100 g/ml). When S. marcescens isolates were grown at 45C cured and sensitive bacterial colonies were observed at a frequency of 10–94%. These, however, were resistant to Erythromycin. Treating the isolates with Acridine orange cured them of all them resistance except Erythromycin at 10–92% .Urea treatment cured their of all resistance phenotypes except Ampicline and Chloramphenicol , also at a frequency of 10–93%. | ||
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