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Study on Selectivity of Acriflavine Dye Added to Some Culture Media for Cultivation of Klebsiella Pneumoniae Isolated from Different Sources | ||
| Rafidain Journal of Science | ||
| Article 24, Volume 16, Issue 14, 2005, Pages 251-257 PDF (0 K) | ||
| DOI: 10.33899/rjs.2005.43536 | ||
| Authors | ||
| Soubhi H. Khalaf; Mahmood Z. Al-Hasso | ||
| Abstract | ||
| Four culture media were prepared and tested for their ability to support the growth of Klebsiella pneumoniae . The tested media were violet red bile agar , orange leaf agar , essential salts agar and date extract agar. Acriflavine dye was added to all media in 0.01% as a selective material for this bacterium. Seven isolates of K . pneumoniae for different pathogenic cases were used and compared their growth and recovery percentages on the tested media in comparison with nutrient agar, other bacterial species were also cultured for comparison. The results indicated that acriflavine violet red bile agar is the best of the tested media for the cultivation of K .pneumoniae then come acriflavine orange leaf agar.Also acriflavine violet red bile agar showed a good selectivity for this bacterium in comparison with the other species used in the study. | ||
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