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Activity of Adenylate Cyclase in Embryonic Cell | ||
| Rafidain Journal of Science | ||
| Article 28, Volume 16, Issue 14, 2005, Pages 290-298 PDF (0 K) | ||
| DOI: 10.33899/rjs.2005.43576 | ||
| Author | ||
| Saad G. Salih | ||
| Abstract | ||
| A total of 10 normal cases for each pregnancy month, from the fifth month, were randomly selected, in Mosul Albatool hospital. Also 3 cases of abnormal embryos were chosen for each month, from the seventh month of pregnancy. Amniotic fluids was collected, by trans-vaginal method amniocentesis. The embryonic cells were separated and extracted; the resulted pellets were used for adenylate cyclase determination. While cyclic AMP measured in both cell filtrate and the amniotic fluids. The result yield that cyclic AMP decreases, from 50 μg /l. to 17 μg/l. in amniotic fluid, and from 32μg/l. to 16 μg/l. in embryonic cell through the last three month of pregnancy. Also the results showed an increase in cyclic AMP, in abnormal pregnancy comparing with the normal cases while a decreases through pregnancy advancing in the last three month in both amniotic fluid and embryonic cell from 62μg/l to 59μg/l and from 44μg/l to 42μg/l respectively. While the activity of adenylate cyclase decrease in relative to the cyclic AMP level, yielding 32.234 μmol/min/mg enzyme to 11,231μmol/min/mg enzyme, in normal embryonic cell and from 22.245 μmol/min/mg to 21.231 μmol/min/mg enzyme in abnormal embryonic cell. While protein did not change significantly in embryonic cell in both normal and abnormal cases. | ||
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