H. Al- Khateeb, E., J. Aawad, Z. (2006). Using Tissue Culture Techniquefor the Production of Cardiac Glycosides From Roots of Digitalis purpurea L Plantlets( Var .Excelsior Mixed ). , 15(1), 92-98.
Ekbal H. Al- Khateeb; Zeinab J. Aawad. "Using Tissue Culture Techniquefor the Production of Cardiac Glycosides From Roots of Digitalis purpurea L Plantlets( Var .Excelsior Mixed )". , 15, 1, 2006, 92-98.
H. Al- Khateeb, E., J. Aawad, Z. (2006). 'Using Tissue Culture Techniquefor the Production of Cardiac Glycosides From Roots of Digitalis purpurea L Plantlets( Var .Excelsior Mixed )', , 15(1), pp. 92-98.
H. Al- Khateeb, E., J. Aawad, Z. Using Tissue Culture Techniquefor the Production of Cardiac Glycosides From Roots of Digitalis purpurea L Plantlets( Var .Excelsior Mixed ). , 2006; 15(1): 92-98.

Using Tissue Culture Techniquefor the Production of Cardiac Glycosides From Roots of Digitalis purpurea L Plantlets( Var .Excelsior Mixed )

Iraqi Journal of Pharmaceutical Sciences
Article 1, Volume 15, Issue 1, 2006, Pages 92-98
Authors
Ekbal H. Al- Khateeb; Zeinab J. Aawad
Abstract
The present study was performed using the tissue culture technique , where by roots formation was stimulated shoot tips of Digitalis purpurea L plant using auxins in this study ,shoot tips (1cm length ) were excised from sterilized seedlings of Digitalis purpurea L.Var Excelsior mixed , then they were cultured on MS medium with the addition of one of the auxins IAA, IBA ,NAA of different concentration ( 0.0 ,0.05 ,0.1 ,0.5 and 1.0 mg / L ).After 45 days of starting the culture results showed that the treatment 0.5 mg/ L of IBA had a high significant effect on the dry weight of roots formation and containing Digitoxin and Gitoxin whose quantities as a rate of ( 2.96 gm , 30.01 and 11.05 ƒفg/gm dry weight respectively .Also this treatment has given highest values with the other studied characteristics where the percent of the soluble sugars and starch and they are ( 2.48 % , 2.82%) respectively Abbreviation (Indole Butyric Acid )IBA , ( Indole Acetic Acid )IAA , ( Murashige and skoog medium , 1962 ) MS , (Naphthalen acetic Acetic ) NAA , ( Milligram ) Mg , ( Microgram )ug , (Litter ) L , (Gram) gm .
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