Sinan Abdulsattar, R. (2012). Spectrophotometric Determination of Nitrazepam in Pharmaceutical Tablets Using Flow Injection Analysis. , 4(1), 40-45. doi: 10.37652/juaps.2010.43885
Raghad Sinan Abdulsattar. "Spectrophotometric Determination of Nitrazepam in Pharmaceutical Tablets Using Flow Injection Analysis". , 4, 1, 2012, 40-45. doi: 10.37652/juaps.2010.43885
Sinan Abdulsattar, R. (2012). 'Spectrophotometric Determination of Nitrazepam in Pharmaceutical Tablets Using Flow Injection Analysis', , 4(1), pp. 40-45. doi: 10.37652/juaps.2010.43885
Sinan Abdulsattar, R. Spectrophotometric Determination of Nitrazepam in Pharmaceutical Tablets Using Flow Injection Analysis. , 2012; 4(1): 40-45. doi: 10.37652/juaps.2010.43885

Spectrophotometric Determination of Nitrazepam in Pharmaceutical Tablets Using Flow Injection Analysis

Journal of University of Anbar for Pure Science
Article 14, Volume 4, Issue 1, 2010, Pages 40-45    PDF (257.64 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2010.43885
Author
Raghad Sinan Abdulsattar*
University of Baghdad - College of Science
Abstract
Nitrazepam (NZP) was determined spectrophotometrically in the pure form and in the
pharmaceutical tablets using flow injection analysis (FIA). The method was based on oxidative coupling organic
reaction of reduced NZP with promethazine hydrochloride in the presence of sodium periodate to give a green
solution having an absorbance maximum at 613 nm. The various chemical and physical variables were optimized.
The calibration graph of NZP is linear from 1 to 50 μg mL-1 with detection limit (S/N = 3) was 0.9284 μg mL-1.
The method was successfully applied to the analysis of NZP in pharmaceutical tablets. The results obtained by
applying the proposed FIA method were in good agreement with those obtained by British Pharmacopoeia method
at the 95% confidence.
Keywords
nitrazepam; Promethazine hydrochloride; Pharmaceutical Tablets; Flow injection analysis
References
  1. “British Pharmacopoeia”, (2001). 303, 832, The Stationery Office, London.
  2. B. G. Katzung, (2007). “Basic and Clinical Pharmacology”. 475, 10th Ed., The McGraw-Hill Companies, Inc., USA.
  3. A. M. Rubio, J. V. J. Ortiz, A. Salvador, and M. D. L. Guardia, (1994). Hydrolysis of benzodiazepines in a microwave oven and ultraviolet derivative analysis of their benzophenones. Microchem. J., 49 (1): 12-19.
  4. M. Kleinschnitz, M. Herderich, and P. Schreier, (1996). Determination of 1,4-benzodiazepines by high-performance liquid chromatography-electrospray tandem mass spectrometry. J. Chromatogr. B, 676 (1): 61-67.
  5. M. Tomita, and T. Okuyama, (1996). Application of capillary electrophoresis to the simultaneous screening and quantitation of benzodiazepines. J. Chromatogr. B, 678 (2): 331-337.
  6. R. I. Allen, K. J. Box, J. E. A. Comer, C. Peake, and K. Y. Tam, (1998). Multiwavelength spectrophotometric determination of acid dissociation constants of ionizable drugs. J. Pharm. Biomed. Anal., 17 (4-5): 699-712.
  7. M. E. C. Peiró, D. Bose, A. M. Domínguez, M. G. Agustí, and J. E. Romero, (2002). Direct injection micellar liquid chromatographic determination of benzodiazepines in serum. J. Chromatogr. B, 780 (2): 241-249.
  8. M. Bakavoli, and M. Kaykhaii, (2003). Quantitative determination of diazepam, nitrazepam and flunitrazepam in tablets using thin-layer chromatography-densitometry technique. J. Pharm. Biomed. Anal., 31 (6): 1185-1189.
  9. T. Gunnar, K. Ariniemi, and P. Lillsunde, (2005). Determination of 14 benzodiazepines and hydroxyl metabolites, zaleplon and zolpidem as tert-butyldimethylsilyl derivatives compared with other common silylating reagents in whole blood by gas chromatography-mass spectrometry. J. Chromatogr. B, 818(2): 175-189.
10. K. C. Honeychurch, G. C. Smith, and J. P. Hart, (2006). Voltammetric behavior of nitrazepam and its determination in serum using liquid chromatography with redox mode dual-electrode detection. Anal. Chem., 78 (2): 416-423.

11. S. R. El-Shabouri, (1986). Spectrophotometric determination of nitrazepam in tablets. Talanta, 33 (9): 743-744.

12. M. I. Walash, M. Rizk, and A. El-Brashy, (1988). Spectrophotometric determination of chlordiazepoxide and nitrazepam. Talanta, 35 (11): 895-898.

  1. 13.  S. M. Hassan, F. Belal, M. S. El-Din, and M. Sultan, (1988). Spectrophotometric determination of some pharmaceutically important nitro compounds in their dosage forms. Analyst, 113 (7): 1087-1089.
14. R. S. Abdul Satar, (2009). Spectrophotometric determination of nitrazepam in pharmaceutical tablets. Iraqi J. Biotech, 8 (1): 252-262.

15. R. Sinan, and M. Q. Al-Abachi, (2009). Spectrophotometric determination of nitrazepam in pharmaceutical tablets by oxidative coupling reaction with pyrocatechol. J. of University of Anbar for Pure Science, 3 (3): 6-12.

16. E. Ruiz, M. H. Blanco, E. L. Abad, and L. Hernández, (1987). Determination of nitrazepam and flunitrazepam by flow injection analysis using a voltammetric detector. Analyst, 112 (5): 697-699.

17. J. Dolejs'ova´, P. Solich, Ch. K. Polydorou, M. A. Koupparis, and C. E. Efstathiou, (1999). Flow-injection fluorimetric determination of 1,4-benzodiazepines in pharmaceutical formulations after acid hydrolysis. J. Pharm. Biomed. Anal., 20 (1-2): 357-362.

J. N. Miller and J. C. Miller, (2001). “Statistics and Chemometrics for Analytical Chemistry”. 116, 117, 121, 47, 52, 4th Ed., Pearson Education Limited, London.

Statistics
Article View: 477
PDF Download: 402


Journal Management System. Powered by iJournalPro.com