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Local Isolate of Bacillus stearothermophilus Producing Alkaline Phosphatase Activity by two Fermentation Systems | ||
| Iraqi National Journal Of Chemistry | ||
| Article 1, Volume 0, Issue 17, 2014, Pages 143-150 | ||
| Authors | ||
| Haider Mussa Hamza; Hamid Ghafory Hassan | ||
| Abstract | ||
| Abstract From soil samples in different region of Iraqi-Kurdistan, thirty-one isolates of Bacillus spp. were isolated and identified in all samples according to morphology of colonies, cells and their growth at 55°c. Using high temperature 75°c and some biochemical tests, only seven isolates were diagnosed as B. stearothermophilus. The isolate no. 18 was selected based on its high activity of enzymes (amylases & proteases). Using two fermentation systems (liquid state & solid state fermentation), the isolate no.18 was examined for production of alkaline phosphatase (AP) enzyme activity. The liquid state fermentation method for this isolate gave a 4.9 un it/ml enzyme activity at optimum pH and 5.8 unit/mg protein specific activity, whereas the solid state fermentation (wheat bran) gave 11.32 unit/ml enzyme activity and 7.48 unit/mg protein specific activity. Results revealed high activity of alkaline phosphatase screening from local isolate of Bacillus stearothermophilus using wheat bran in solid state fermentation. | ||
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