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CONVERSION OF MUCOID PHENOTYPE OF P.AERUGINOSA INTO NON MUCOID AND VISE VERSA AND ITS DETECTION MOLECULARLY | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 10, Issue 1, 2011, Pages 19-32 | ||
| Abstract | ||
| ABSTRACT Study was performed by transformation of mucoid phenotype to non-mucoid phenotype anaerobically and vice versa was done by using 3 mmol. H2O2. Phenolic method was used for DNA extraction which was about 5.2-6.3 μg/μl and with high degree of purity (1.7-2.0). After that, initial detection of DNA extract was done through agarose electrophoresis and showed clear DNA bands before and after the conversion from mucoid to nonmucoid phenotype and vice versa. PCR products for algT, mucA and mucB genes showed whether the mutation has occurred or not indicating on gene binding to its specific primer | ||
| Keywords | ||
| aeruginosa; Mucoid phenotype; Polymerase chain reaction PCR; algT; mucA; mucB | ||
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