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ISOLATION AND SCREENING OF SOME PECTINASES PRODUCTION MOLDS BY SOLID STATE FERMENTATION | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 10, Issue 1, 2011, Pages 41-54 | ||
| Abstract | ||
| ABSTRACT Eleven local fungal isolates of pectinase producing molds have been isolated from different sources included soil, citrus, orange and bread .the fungal isolates were grown on corn cobs hydrated with modified Czepek-Dox media as a solid substrate fermentation medium containing pectin instead of glucose as carbon source, and using corn cobs as a supporting material. The isolates were transferred to Czepek-Dox agar media with pectin to check the ability of growth and pectinase production. Spore suspension of each isolate was prepared, filtered through aseptic filter unit and counted their numbers by using Haemocytometer slide. Then they were identified to genus according to classification keys. Qualitative and quantitative screenings were achieved for election the most pectinase producer isolates, N1 and O2 isolates which belong to Aspergillus were elected, their specific activity were 3133.3, 26470.58 U/mg respectively. | ||
| Keywords | ||
| Pectinases Production; Solid state fermentation; aspergillus | ||
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