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Antiproliferative Activity of Spironolactone: In Vitro Study | ||
| Iraqi Journal of Cancer and Medical Genetics | ||
| Article 1, Volume 4, Issue 2, 2011, Pages 17-23 | ||
| Authors | ||
| Amena Jassim Lafta; Kassim Hassoon Ali; Nahi Y. Yaseen; Mohammed A. Taher | ||
| Abstract | ||
| Spironolactone SPL stock solution was studied against two cancer cell lines Hep-2, and AMN-3. These cell lines were exposed to different concentrations of SPL ranged from 0.0019 to 1000 μg/ml for 24, 48 and 72 hours durations. The optical density was measured under wavelength 492 nm with micro-ELISA reader after their staining with crystal violet dye. The results revealed a clear cytotoxic activity of pure SPL with high significances in a two cancer cell lines during the three exposure time, suggesting that the cytotoxic effect of pure spironolactone is a dose and time dependant. The best cytotoxic activity was on AMN-3 and Hep-2 cell lines at the high concentrations 1000, 500, 250, 125μg/ml. The results also suggest that AMN-3 cell line is the most sensitive cancer cell line to pure SPL than Hep-2 cell line after 24 hr. of exposure to drug. | ||
| Keywords | ||
| Spironolactone SPL; AMN | ||
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