- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Extraction, Purification and Physicochemical Properties of Carcinoembryonic Antigen (CEA) | ||
| Iraqi National Journal Of Chemistry | ||
| Article 1, Volume 0, Issue 34, 2014, Pages 330-346 | ||
| Authors | ||
| Sami A. Al-Mudhaffar; Sahib A. Al-Atrakchi | ||
| Abstract | ||
| Abstract A procedure has been described for the purification of the CEA of the human mammary carcinoma. Tumor tissue extraction in (0.6) M perchloric acid followed by Ion exchange and column chromatography on Sepharose CL–6B column resulted in highly purified CEA preparation as determined by both immunological and physico-chemical criteria. Such as, immunoelectrophoresis, stocks radius, isoelectric point, M.wt., stability and carbohydrate content. The collected data suggest that CEAs of mammary adenocarcinoma tissues with stocks radius (53 A°), pI (4) and the M.wt. have been found to be 175 KD. Finally purified CEA preparation gave rise to single band by disc SDS – polyacrylamide gel electrophoresis and a single preciptin arc against corresponding monoclonal anti–CEA on immunoelectrophoresis. The purified CEA contained approximately 40% carbohydrate through determination by phenol – sulfuric acid method. | ||
|
Statistics Article View: 98 PDF Download: 64 |
||