Propagation of ( Gladiolus hybrida ) cv. Priscilla by shoots production and multiplication in vitro

	Propagation of ( Gladiolus hybrida ) cv. Priscilla by shoots production and multiplication in vitro
journal of kerbala university
Article 1, Volume 5, Issue 1, 2009, Pages 169-174 PDF (0 K)
Abstract
An experiment was conducted at tissue culture laboratory belong to private sector company which located at Abu-Alkhasib Basrah Iraq, from September 2003 to December 2004 .The objectives of the experiment were to test the effect of many treatments on response of gladiolus corm slices cv. Priscilla in vitro culture .The experiment included 14 treatments which were a combination between ammonium nitrate at 0.825 gm/l and 1.65 gm/l with coconut milk 10% and 20% and Benzyl adenine 0.5 mg/l .A combination also made between Naphthalene acetic acid 1 mg/l and ammonium nitrate and coconut milk .Treatments of shoot multiplication medium included four concentrations of Benzyl adenine which were : 0 , 0.5 , 1 , 1.5 , mg/l . It could be suggested. Best response of corm slices was to that cultured on MS medium supplemented with coconut milk 10% and Benzyl adenine at 0.5 mg/l , its response was 80%. The response was the production of adventitious bud on slice. Benzyl adenine treatment of 1 mg/l caused the highest multiplication rate which were 5.2 shoots after six weeks and 17.2 shoots after twelve weeks.The lowest concentration of benzyl adenine ( 0.5 mg/l ) increased shoot length after 6 and 12 weeks of culture which was 10.25 cm and 15.81 cm respectively, whereas , The highest concentration of benzyl adenine ( 1.5 mg/l ) reduced shoot length.

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