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THE GENETIC DISTANCE OF ROSA SPP. USING RAPD | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 42, Issue 2, 2011, Pages 71-79 | ||
| Author | ||
| Janan K. Hussein | ||
| Abstract | ||
| The study was carried out at biotechnology laboratories- GCSAR- Syria in summer season-2010. The random amplified polymorphic DNA (RAPD) based on Polymerase Chain Reaction (PCR) with ten primers were applied, used to estimate fingerprinting and Genetic Distance for six cultivars of Rosa spp. (Tea R., Musk R. , Hybrid perpetuls R. , Climber R. , Miniature R. , French R.). Phases of work included the isolation and purification of DNA plant parts and DNA polymorphisms were scored within amplified fragements by electrophoresis. The results of RAPD analysis were clear differences in the number of DNA amplified fragments and molecular weights depending on the primers user . A total number of polymorphic amplified 68 fragments for all primers used . Genetic Distance for rose cultivars genetic variability found in it. High Genetic Distance Rose cultivars (40.3%) in RAPD markers was registered between the two cultivars (Hybrid perpetuls R. X Climber R.) and lowest Genetic Distance (18.9%) between (Tea R. X Climber R.). Rose cultivars gave genetical and morphological variations confirmed by genetic distance. High genetic differences between rose cultivars could be attributed to the geographical locations they were grown as well as plant breeding made to it. | ||
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