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PRODUCTION OF PECTINASE FROM ASPERGILLUS NIGER BY USING SOME FRUIT PEELS | ||
| Diyala Agricultural Sciences Journal | ||
| Article 1, Volume 4, Issue 1, 2012, Pages 257-267 | ||
| Authors | ||
| Elham Esmael Al-Shamary; Badaa Hatam Kadem | ||
| Abstract | ||
| Ten filamentous fungi isolated from a growaste samples .Three isolated were of high productivity for pectinase enzyme compared with other isolates , depending on diameter of clear hydrolyzed zones on the medium plates containing commercial pectin as sole carbon source , this isolates were Aspergillusniger (A2),closely followed byAspergillus sp.(A1 ) and penicillum sp.(P1) . The three isolates also produced pectinases with different a growastes ( Orange peels , Apple peels , Banana peels)as the sole carbon source ,Aspergillusniger(A2) was the best isolate for pectinase production on the medium containing orange peels as the sole carbon source . Peak pectinase activity of 675 and 370 u/mg protein was respectively obtained by solid – state fermentation (ssf) and submerged fermentation (smf) for A.niger(A2) .Solid – state fermentation yielded higher levels of pectinase activity than the submerged fermentation . The strain of A.niger(A2) have good prospect for pectinase production ,and the orange peels is a good low – cost fermentation substrate for pectinase production by the investigated isolate | ||
| Keywords | ||
| pectinase; Aspergillus niger | ||
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