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Regeneration of date palm (Phoenix dactylifera L.) CV. Sherafy from different apical explants In vitro | ||
| Basrah Journal For Date Palm Research | ||
| Article 6, Volume 6, Issue 1, 2007, Pages 64-80 PDF (0 K) | ||
| Author | ||
| Khaun Ali Muhsen | ||
| Abstract | ||
| This study has been performed to determine the response of apical sources [shoot tip ; Auxillary buds ; sub apical tissues ; leaf primordial and flower buds] when cultivated on MS medium supplemented with 30 mg/L of NAA ; 3 mg/L of 2ip and 3 g/L activated charcoal in inducing ; formation of callus and production of somatic embryos on the same medium enriched with 10gm/L of NAA ; 2 mg/L of 2ip and 2 g/L activated charcoal and formation organogenesis from callus on two media which were supplemented with 3 mg/L 2ip ; 1 mg/L 2ip and 1 mg/L NAA ; 250 mg/L charcoal for each media. Results revealed that the flower buds surpassed in the time requested for callus estabelish ment and the plant sources ratio which produced the callus, with significant difference than other explant, the sub apical tissue failed to produce the callus also, the shoot tip treatment had the highest average of produced callus than other explant. Statistical analysis proved that the difference between flower buds and shoot tip was not significant in the parameter of the dry weight of callus. Which cultivated in ms medium supplemented with 10 gm/L of NAA after two months from culturing, while the dry weight decreased significantly in leaf primordial than other explant. Flower buds treatment had the optimam periode to estabelish the cylinderial embryos and number in comparision with other explant while the longest periode recorded in leaf primordial treatment. Resulted showed that the callus cultured on the medium supplemented with 3 mg/L of 2ip decreased significantly in the requested periode for organogenesis growth which were (186 and 213.25) days respectively. The interaction between (shoot tip and 3 mg/L of 2-ip) was surpassed the explant of shoot tip and 1 mg/L of 2ip in the requested time for appearance of organogenesis, also some individual cylindrical somative embryos developed from the callus cultured on the above media with some secondary embryos when cultured on the same medium produced muttistem plantlets . | ||
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