STUDY OF ANTIMICROBIAL ACTIVITY OF SALIX

	STUDY OF ANTIMICROBIAL ACTIVITY OF SALIX
Iraqi Journal of Biotechnology
Article 1, Volume 7, Issue 1, 2008, Pages 38-50
Abstract
ABSTRACT Antimicrobial activity of cold aquous and ethanol extracts with six concentrations of each one (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) mg/ml of Salix acmophylla leaves were estimated against four types of pathogenic bacteria (E. coli, S. aureus, Klebsiella spp., St. pyogenes) after incubation at (37)oC for (24)hr. by using well diffuion assay method. Antimicrobial activity of both extracts was compared with that for a number of antibiotics include Ampicilin, Cephalexin, Erythromycin, Amoxicilin, Gentamicin and Tetracyclin, by using antibiograme test. Both extracts of Salix acmophylla showed clear antimicrobial activity against pathogenic bacteria. This activity was enhanced with the increasing of extracts concentrations. The 0.6% concentration of both extracts gave highest activity against E. coli, S. aureus and St. pyogenes with no antimicrobial activity against Klebsiella spp. E. coli was the least testing bacteria affective by the concentrations of both extracts. While S. aureus was the most effective one by the concentrations of aquous extract and St. pyogenes was the most affective one by the concentrations of ethanolic extract. Antimicrobial activity of cold aquous and ethanolic extracts of Salix acmophylla was better than that to Gentamicin against St. pyogenes and S. aureus, while the inhibition effect of Tetracyclin was better than both extracts concentrations against S. aureus and E. coli. The inhibition effects of Gentamicin and Erythromycin and both latest two concentrations of both extracts against E. coli were closely related. Klebsiella spp. was sensitive to Gentamicin only. A resist of pathogenic bacteria to Ampicillin, Amoxicilin and Cephalexin was observed.
Keywords
Antimicrobial activity; Salix acmophylla extracts; Pathogenic bacteria

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