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STUDY THE EFFECT OF LACTOBACILLUS AS | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 7, Issue 1, 2008, Pages 64-73 | ||
| Abstract | ||
| ABSTRACT This study included to investigate the types of Lactobacilli which found in the milk of 35 nursing mothers, and study the effects of these normal friendly bacteria on Candida albicans. Eleven species of the genus Lactobacillus were isolated and distributed as ; thirteen isolate belong to L. plantarum (26 %) , (12) isolate to L. gasseri (24 %) , while L. acidophilus represent (6) isolates (12%) , L. fermentum(5) isolates (10%) , L. casei (3) isolates (6 %) , beside 2 (4 %) isolates for each of L. lactis , L. rafinotactis , L. paracasei , L. rhamnosus , and L. crispatis and only one isolate belongs to L. salivaris (2%) . Candida albicans was diagnosed in 25 samples of saliva from infants affected by thrush. The susptibility of C. albicans was tested against five antifungal drugs The results revealed that the isolates were sensitive to Miconazole , Ketoconazole and Clotrimazol , the zone of inhibition reached to 11, 10 and 10 mm. respectively ; on the contrary, the isolates were resistance to the Nystatin and Grisofulvin . The effect of Lactobacillus spp. filtrate were examined against C. albicans and the results were clarified that there was inhibition activity of L. acidophilus and L. fermentum against it, with zone of inhibition reached to (28), (15) mm. respectively. When the effect of Lactobacillus spp. extract on C. albicans DNA was measured , by spectrophotometer at different wave lengths ( 260, 280, 361 nm ) respectively they showed increase of the absorbance with increasing of the amount of extract , which would mean that the extract cause distraction to the DNA of the C. albicans . | ||
| Keywords | ||
| Lactobacilli; Candida Albicans; breast milk; Lactobacillus | ||
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