STUDY OF INHIBITORY ACTIVITY OF VOLATILE OIL STUDY OF INHIBITORY ACTIVITY OF VOLATILE OIL

	STUDY OF INHIBITORY ACTIVITY OF VOLATILE OIL STUDY OF INHIBITORY ACTIVITY OF VOLATILE OIL
Iraqi Journal of Biotechnology
Article 1, Volume 7, Issue 1, 2008, Pages 74-85
Abstract
ABSTRACT Volatile oil was extract from Matricaria chamomilla and Anthemas nobilisd flowers using clavenger devise which was 0.3% and 0.7% for first and second type respectively. The inhibition activity of volatile oil was studied using agar well diffusion method on some pathogenic microorganisms like Escherichia coli, Staphylococcus aureus and Candida albicans and the result showed that the volatile oil had an inhibitory effect on the growth of these microorganisms. The minimum inhibitory concentration (MIC) value for E. coli and St. aureus was (2.5)%and(0.625)% respectively and (2.5)% for C. albicans.while the minimum bactericidal concentration (MBC) for E.coli was (5)% Staph.aureus was(1.25)% and C.albicans was(5)%. The chamomile flower powder also showed inhibition activity for these microorganisms and the (MIC) was (0.125),(0.0312),(0.125)gm/ml for E. coli, St .aureus and C. albicans respectively, while the (MBC)was (0.250),(0.061),(0.250)gm/ml ,respectively . It was accomplished in this study also formulation of ointment for external use contain 5% (v/v) volatile oil and ointment contain 20%(w/w) chamomile flowers powder and two formula were evaluated using High Performance Liquid Chromatography (HPLC).
Keywords
inhibitory activity; Chamomiel; Volatile oil; Flower milled; pathogenic bacteria

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