Study of Optimum Conditions for the Cellulose Production Using Aspergillus niger from Date Pits Powder

K. Nueama, Alaa; J. Al-Manhal, Alaa

	Study of Optimum Conditions for the Cellulose Production Using Aspergillus niger from Date Pits Powder
Basrah Journal For Date Palm Research
Article 6, Volume 5, Issue 1, 2006, Pages 71-79 PDF (0 K)
Authors
Alaa K. Nueama; Alaa J. Al-Manhal
Abstract
T he enzyme activity for five fungal isolates from Aspergillus niger growth on a solid substrate was studied and it was found that best isolate ( A5 ) . The optimum conditions for the enzyme production was studied. It was found that the enzyme activity ( 2.8 u/ml ) was obtained at PH 5 as compared with these pH ( 3 , 4 , 6 and 7 ) . while that optimum temperature at 22 Cº as the enzyme activity increased ( 2.3 u/ml ) Compared with these ( 18 , 20 , 24 , 26 and 28 Cº ) and the optimum incubation time was 8 days as the enzyme activity increased from 0.65 to 3.3 u/ml after ( 2, 4 , 6 and 8 days ) of incubation . The optimum conditions was using for production cellulase from substitution a cellulosic solid substrate as date pits powder for the growth at levels ( 10 , 20 , 30 , 40 , 50 , 60 , 70 and 80 % ) and best substitution at 50 % it was found that hydrolysis diameter 4.2 cm and the enzyme activity ( 3.4 u/ml ) .

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