Effect Of Amino Acid Cysteine And Its Derivative Cystine On Growth Of Callus And Formation Of Somatic Embryos In Date Palm Tissue Culture Phoenix dactylifera L. cv. Ashkar

A. M. Al-Abadi, Usama

	Effect Of Amino Acid Cysteine And Its Derivative Cystine On Growth Of Callus And Formation Of Somatic Embryos In Date Palm Tissue Culture Phoenix dactylifera L. cv. Ashkar
Basrah Journal For Date Palm Research
Article 11, Volume 5, Issue 1, 2006, Pages 137-149 PDF (0 K)
Author
Usama A. M. Al-Abadi
Abstract
T he study was conducted at tissue culture laboratory – date palm research center – basrah university , to elucidate the effect of addition of amino acid cysteine and its derivative cysteine at concentrations 0 , 25 ,75 , 125 and 250 mg /l on growth of embryogenic callus and development of somatic embryos for date palm cv. Ashkar in (MS) Murashige and Skoog medium . Fresh and dry weight , number of somatic embryos and its browning percentage were determined as marker for effect of these two amino acids on callus tissue . Results showed that cystine at conc. 125 mg /l gave the highest fresh and dry weights of callus which were 1.57 and 0.158 gm , respectively , while there was no significant difference between cysteine’s treatments and control (0 mg /l) . In addition , cystine at conc. 75 mg /l showed highest quantity of somatic embryos which was 7 with significant difference from other treatments, while There was no significant difference between cysteine’s treatments and control. The study showed that cysteine at concentrations 25, 75 and250 mg /l gave the least browning percentage of somatic embryos with significant difference from control ,while there was no significant difference between cystine’s treatments and control. Thus , the results showed that cystine stimulated growth of callus and formation of somatic embryos better than cysteine , while cysteine inhibited the browning better than cystine .

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