Acclimation plantlets of the two cultivars of date palm (Phoenix dactylifera L.)By in vitro

N.J Al-meer, Usama; Sami Jameel, Nael

	Acclimation plantlets of the two cultivars of date palm (Phoenix dactylifera L.)By in vitro
Basrah Journal For Date Palm Research
Article 1, Volume 10, Issue 1, 2011, Pages 1-17 PDF (0 K)
Authors
Usama N.J Al-meer; Nael Sami Jameel
Abstract
This study was conducted in the laboratory of plant tissue culture research center of the palm / University of Basra to the production of plantlets acclimatized and subsequently for the two types of date palm is rare in Basra (Barhi and Kantar) through the formation of buds spin-off of the initial callus. Took fourths of terminal buds Shoot tips of 3-year-old cuttings and planted in the media of diet and MS the forces of steel and supplied complete with 30 mg / L naphthalene acetic acid NAA and 3 mg / L 2ip and 3 g / liter of activated charcoal, 30 gm / liter of sucrose and added e vitamins Biotin and Thiamin-Hcl Pyridoxine at a concentration of 1 mg / L adenine sulphate was also added of close concentration of 40 mg / L and sodium ortho phosphate acid concentration of 170 mg / L and 100 mg / L of the Meso-inosetol and add the Agar concentration of 7 g / L and incubated at growth chamber on temperature of , the 27c±2 intensity of light 1000 Lux and 16 hours after light and 8 hours darkness and were secondary Agriculture Sub-Culture every 4 weeks until the callus was then changed on the medium of for the purpose of stimulating the Buds incidental. Results of the study have shown the following: 1-The use of plant growth regulator Naphthalene acetic acid at concentration of 30 mg / L had an emotional impact in the rate of weight and time required for the initial appearance of callus, callus may be the first in a class Barhi after 102 days, while Kuntar be in a class after 72 days, as The average weight of callus after 8 weeks for up to 406 and 420 mg of Barhi Qantar, respectively, when using the focus 40 mg L and without focus on the moral difference 30 mg / liter. 2-It was noted that the use of concentration 2 mg / L of naphthalene acetic acid with 6 mg / L 2ip had an emotional impact in reducing the time required for the emergence of spin-off buds, buds, and formed a spin-off in class Barhi 134.10 after day, when they disagreed with the term class Kuntar was formed as buds off after 94.18 the day when the use of 1 mg / L of naphthalene acetic acid with 4 mg / L 2ip . 3-After a protracted process of buds off and have been rooting plantlets acclimatization in the laboratory and the results of research have shown that the use of agricultural medium component of sand and peat moss by 1:2 led to a significant increase in the percentage of plantlets adapted amounted to 70 and 90% Barhi Qantar, respectively, while the percentage dropped to 30 and 40% when you use the agricultural medium component of sand only Barhi Qantar, respectively. 4-clear from the results that plantlets have been obtained for the class Kuntar is a better and stronger than those of class Barhi as strongly marked by its leaves and not to lose moisture quickly with those sorts of Barhi, and the plantlets class Kuntar has been characterized to possess a thick roots and the roots of the secondary container.

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