- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
DETERMINATION OF PLASMIDS ROLE IN BACTERIOCIN PRODUCTION FROM PATHOGENICKLEBSIELLA | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 10, Issue 2, 2011, Pages 227-241 | ||
| Abstract | ||
| One hundred and twenty five sample were collected as(50 Burne+wound and75 blood samples)from hospital patients (Ibn Albalady and Alkindy Hospital in Baghdad at period Nov .2010. to March. 2011). 40 bacterial isolates were isolated of which 18 isolates showed similar characteristic to Klebsiella depending on phenotypic characterization and biochemical test and using with AP1 20E system and VITEK 2. Agar disc were used to screen the isolates for their ability of bacteriocin production. The result showed that7isolates had the ability of bacteriocin production specially Klebsiella terrigena that show the highest production. Plasmid content was studied for Klebsiella isolates .Results showed that some isolates has plasmid and genomic DNA. To determine the role of plasmid in bacteriocin production, two highest isolates were selected (Klebsiella terrigena 7 and Klebsiella terrigena 6 )to perform plasmid curing experiment using Ethidium bromide. Result showed that the plasmid has been cured at 512 mg /ml Ethidium bromide. the cured cells lost their ability of bacteriocin production which proved the bacteriocin gene is located on plasmid. To confirm this result, transformation experiment was performed by transferring the plasmid the extracted from Klebsiella terrigena 7 to the standard strain E.coli MM 294rif + which is free of plasmid and have no resistance to Ampicillin (Amp), the result showed that, the E.coli MM 294rif + strain become Amp resistant in addition it had the ability to produce the bacteriocin. | ||
| Keywords | ||
| Klebsiella; Bacteriocin | ||
|
Statistics Article View: 38 PDF Download: 13 |
||