Brilliant Blue- Non Mercuric Schaudinn′s Fixative Solutions Wet Mount for Identification of Intestinal Parasites in Stool Specimens

Kassim Hasan, Yahya; Abdual Sattar Al-Janabi, Shahrzad

	Brilliant Blue- Non Mercuric Schaudinn′s Fixative Solutions Wet Mount for Identification of Intestinal Parasites in Stool Specimens
Iraqi Postgraduate Medical Journal
Article 1, Volume 9, Issue 3, 2010, Pages 244-256 PDF (0 K)
Authors
Yahya Kassim Hasan; Shahrzad Abdual Sattar Al-Janabi
Abstract
ABSTRACT: BACKGROUND: Definitive diagnosis of the intestinal parasites requires the demonstration of the organisms or eggs in feces or tissues. Stool specimens should be preserved , stained and microscopically examined. METHODS: Copper sulfate schaudinns fixative ( CuSO4SF ) solution and zinc sulfate schaudinns fixative ( ZnSO4SF ) solution were prepared, 2 sets of 5 concentrations (serially diluted ) were prepared first set Brilliant blue food color powder (B) was dissolved in CuSO4SF solution ( B-CuSO4SF ) solution second set Brilliant blue food color powder was dissolved in ZnSO4SF solution ( B-ZnSO4SF ) solution Merthiolate-iodine-formalin ( MIF ) preservative and staining solution (control solution) was prepared.  During one year 85 positive stool samples were collected from patients complaining gastro- intestinal tract disorder.  Stool-CuSO4SF suspension, stool-ZnSO4SF suspension and stool-MIF suspension were prpared from each stool sample.  Eleven slides were prepared from each stool sample and examined microscopically by wet mount technique,5 slides were prepared from each concentration, on a slide one drop from B-CuSO4SF and one drop from stool- CuSO4SF suspension were mixed. Another 5 slides were prepared from each concentration, on a slide one drop of B-ZnSO4SF and one drop from stool -ZnSO4SF suspension were mixed. One wet mount smear was prepared from one drop of stool-MIF suspension ( control test ). RESULTS:  Both solutions B-CuSO4SF and B-ZnSO4SF were showed similar results, these two solutions were very efficient in preserving and staining trophozoites and cysts of intestinal protozoa, helminth eggs and non parasitic findings in the wet smear of stool specimen during 24h.  The most appropriate concentration for two solutions were 0.2%wt/vol B-CuSO4SF and 0.4%wt/vol B-ZnSO4SF  During one year B-CuSO4SF and B-ZnSO4SF were very efficient in staining of the intestinal protozoa and helminth eggs. CONCLUSION: Both 0.2%wt/vol B-CuSO4SF and 0.4%wt/vol B-ZnSO4SF solutions may be considered as an efficient one step rapid, safe and economical stain of wet mount stool smear for using in the identification of the intestinal parasites
Keywords
brilliant blue; copper sulfate schaudinns fixative; zinc sulfate schaudinns fixative

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