Effect of MS salts and sucrouse  on formation of lateral buds of date palm cv. Sayer in vitro

Aqil,; A.S.Al-khalifa,; Usama,; N.J.Al-Meer,; M. A. Al-Jabary, Khearallah

	Effect of MS salts and sucrouse on formation of lateral buds of date palm cv. Sayer in vitro
Basrah Journal For Date Palm Research
Article 1, Volume 8, Issue 1, 2009, Pages 39-50 PDF (0 K)
Authors
; ; ; ; Khearallah M. A. Al-Jabary
Abstract
This study was effectuated at Date Palm tissue culture laboratory. (Date Palm Research Center –Basrah University) to study the effect of of MS salts and sucrose on formation of lateral buds of date palm offshoots c.v sayer.The quarter apical buds was used which was obtained from offshots (2-3)years .different concentration of MS salts was teasted (half strength , full strength ,full half strength, dubel strength) and different concentration of sucrose(30,40,50,60) g/l. These explants were cultured on nutrient medium supplemented 1 g/l activated charcoal ,7 g/l agar ,3 mg/l NAA and cytokinins (2iP) 10 mg/l. Cultures were incubated in darkness for four months at 27±1 c0 and sub cultured was done every four weeks then the culture was transfer to lights at 1000 lux at periods16 hours daily the results showed that: 1- Results showed that the use full half strength of MS salts was stimulated to formation of lateral buds in short period was reached(187.6) days and led to have higher number of lateral buds(4.8) compared to the other concentration. 2-Used of 40 g/l sucrose which was reduced the period to formation of lateral buds was reached (190.5) days. significant compared to the other concentration. and led to have higher number of lateral buds(4.8). 3-Results showed that the use full half strength of MS salts and 40 g/l sucrose which was reduced the period to formation of lateral buds was reached (174) days. significant compared to the other concentration. and led to have higher number of lateral buds(7.3).
Keywords
MS salts; sucrouse; lateral buds; date palm

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