Effect of age of offshots and type, supplement time of explants on formation of lateral buds of date palm cv. Sayer in vitro

R.Abdul-Sameed,; Ahmeed,

	Effect of age of offshots and type, supplement time of explants on formation of lateral buds of date palm cv. Sayer in vitro
Basrah Journal For Date Palm Research
Article 1, Volume 8, Issue 1, 2009, Pages 100-110 PDF (0 K)
Authors
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Abstract
This study was effectuated at Date Palm tissue culture laboratory. (Date Palm Research Center –Basrah University) to study the effect of age of offshots and type ,supplement time of explants on formation of lateral buds from quarter apical buds,sub meristemic regon and axillary buds of date palm offshoots c.v sayer. With different ages (1,2,3,4)years. These explants were cultured on nutrient medium of (MS)salts full strength supplemented 30 g/l sucrose,1 g/l activated charcoal ,7 g/l agar ,3 mg/l NAA and cytokinins (2iP) 10 mg/l. Cultures were incubated in darkness for four months at 27±1 c0 and sub cultured was done every four weeks then the culture was transfer to lights at 1000 lux at periods16 hours daily the results showed that: 1-The best age of offshots for tissue cultuer (2 or 3) years which was reduced contamination and browning of explants and formation of lateral buds in few period was reached (168,175) days respectively. 2-Used of the quarter apical buds which was reduced contamination and browning of explants and stimulated to formation of lateral buds in short period was reached (178) days. significant compared to the other explants. 3-The best supplement time of explants for tissue culture was in (october) winter time, which was reduced contamination and browning of explants and formation of lateral buds in few period was reached (168) days. compared to the other supplement time.

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